Tri Forage Horsehae
Tri Forage Horsehae

Research

Tri-Forage Farms Ltd. has a strong belief in, and commitment to, healthy feed for healthy horses. We have demonstrated our confidence in our product over the years by our partnership with the Equine Research Centre and the Nutraceutical Alliance and their research initiatives.

A study by the Equine Research Centre in 2001 found Triforage Horsehae to be clear of mycotoxins. Mycotoxins can cause reproductive, respiratory, gastrointestinal and other disorders in horses and other livestock.

Report: Mycotoxin Analysis

March 2, 2001

By Susan Raymond,
Respiratory Health and Air Quality Research

Background

Mouldy feeds and forage can contribute to a range of disorders in the horse. Moulds and actinomycetes can cause primary allergic and inflammatory respiratory disease, as well as influencing the incidence, severity and duration of episodes of infectious respiratory disease. Mould can also contribute to disorders by the production of mycotoxins. Mycotoxins are metabolites (substances) naturally produced by moulds while in the field or in storage. Mycotoxins can cause reproductive, respiratory, gastrointestinal and other disorders in livestock including the horse.

Methods

Forage samples
Grab samples from 10 bags of each forage product* were pooled and frozen prior to analysis. Three replicates for each analysis were conducted.

*forage products:
Tri-Forage Horsehae (Meadow Mix)
Tri-Forage Horsehae (Alfalfa/Timothy Mix)

Mycotoxin Analysis The concentrations of the mycotoxins; vomitoxin, T2 toxin and zearalenone were analysed using Enzyme-Linked Immunosorbent Assay (ELISA) in the laboratory of Central Lab Services of Ralston Purina Canada Inc.. Briefly, the samples were ground and mycotoxins extracted with distilled water. Each assay was performed with standards containing mycotoxins with known concentrations and a control. Mycotoxin concentrations were calculated by comparing the light absorbencies of the samples with those of known standards (6 % CV).

Results

The results of the mycotoxin analysis are listed below.

Mycotoxin levels (ppm) found in Tri-Forage Meadow Mix (identical levels were found in 3 replicates).

Mycotoxin levels (ppm) found in Tri-Forage Alfalfa/Timothy Mix (identical levels were found in 3 replicates)

Conclusions

No detectable levels of the mycotoxins; vomitoxin, T2 toxin and zearalenone were found in either Tri-Forage Horsehae Meadow Mix or Alfalfa/Timothy Mix of the samples taken.

Other research

A 1994 study by the Equine Research Centre (ERC), “Respirable particles measured when feeding two forage types; Horsehae and dry hay”, clearly demonstrated that the number of dust particles were “significantly lower when Horsehae was fed as compared to dry hay.”